Ovarian tissue preservation is one of the few option for fertility preservation for cancer patients.
For freezing, tissue was cut to slices of size of 1mm x 10mm x 10mm or smaller. These slices were then exposed to a LYO solution composed of DMSO and HSA in a holding buffer medium for an exposure time of 5 to 10 minutes, placed on a carrier or inside a straw having a special pod, plunged into LN or sterile liquid air using FertileSafe’s CLAir device, and then placed into the LyoLife bench top device for primary and secondary drying. Primary drying was conducted at a high sub-zero temperature, slightly lower than the Tg’ of the LYO solution used. Secondary drying was then conducted by increasing the shelf temperature in a step-wise manner. Finally rehydration was achieved by removing the carriers containing the tissue slices and immersed into a warm solution.
No differences were observed between the histology of fresh and freeze dried ovarian tissue.
Further studies is required to see if the freeze dried ovarian tissue can developed into normal oocytes.
Figure 1: Froze-dried mice ovarian tissue